strain background  (TaKaRa)

Journal: Nucleic Acids Research

Article Title: PhoP-regulated VirK acts as an accessory factor to maintain virulence in polymyxin-resistant Klebsiella pneumoniae

doi: 10.1093/nar/gkag290

Figure Lengend Snippet: Transcriptomic analysis of Mut-S in comparison to Kpn2146 strains. ( A ) Volcano map displaying the upregulated (red dots) and downregulated (yellow dots) genes between the Mut-S group and the control group. ( B ) Changes in the relative expression of the mgrB, phoP , and virK genes in the Mut-S strain compared with those in the WT strain. ( C )Changes in the virK gene expression in the Mut-S strain after supplementation with the mgrB gene. ( D ) Changes in phoP and virK gene expressions after phoP gene knockdown in the Mut-S strain. ( E ) Changes in the mgrB and virK gene expressions after the mgrB gene was complemented in the phoP knockout strain in Mut-S. ( F ) Changes in the phoP and virK gene expressions after the phoP gene was supplemented in the phoP knockout strains in Mut-S. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: The effects of virK on bacterial pathogenicity were further evaluated in a mouse systemic infection model using virK mutants and complemented strains under K. pneumoniae ATCC BAA2146 (wild type) or Mut-S ( mgrB truncated strain) backgrounds.

Techniques: Comparison, Control, Expressing, Gene Expression, Knockdown, Knock-Out

Journal: bioRxiv

Article Title: Positive allosteric modulator of SERCA pump NDC-1171 attenuates cardiac functional decline in mouse model of Duchenne muscular dystrophy

doi: 10.64898/2026.03.05.709950

Figure Lengend Snippet: Study design outlining treatment of DBA/2J (control), D2. mdx vehicle, and D2. mdx NDC□1171 groups (n = 9 per group). Mice received oral gavage from 6 to 14 weeks of age. Ultrasound transducer icons denote timepoints at which transthoracic echocardiography wa performed (baseline: 6, midpoint: 11, endpoint: 14, and post-behavior: 16 weeks of age; n□=□5–6 per group). Behavioral testing icon represents the functional behavior testing (treadmill exhaustion and four□limb grip strength) conducted at week 15. Mice were euthanized at week 17 and cardiac tissue was collected for histological evaluation.

Article Snippet: Male D2. mdx mice (n=18, strain #013141, D2.B10-Dmdmdx/J) and its background strain DBA/2J mice (Control) were obtained from Jackson Laboratory at 4 weeks of age (n=9).

Techniques: Control, Functional Assay

Journal: bioRxiv

Article Title: Positive allosteric modulator of SERCA pump NDC-1171 attenuates cardiac functional decline in mouse model of Duchenne muscular dystrophy

doi: 10.64898/2026.03.05.709950

Figure Lengend Snippet: D2. mdx vehicle-treated mice (Vehicle) had impairments when compared to DBA/2J vehicle-treated (Control) in both (A) Four-limb grip strength test and (B) Treadmill exhaustion test. D2. mdx mice treated with NDC-1171 did not improve in either behavior assessments. * p <0.05, ** p <0.01, and *** p <0.001.

Article Snippet: Male D2. mdx mice (n=18, strain #013141, D2.B10-Dmdmdx/J) and its background strain DBA/2J mice (Control) were obtained from Jackson Laboratory at 4 weeks of age (n=9).

Techniques: Control

Journal: Neuron

Article Title: Tachykinin signaling defines distinct populations of glia in the enteric nervous system

doi: 10.1016/j.neuron.2025.11.030

Figure Lengend Snippet: (A) Ileums from 3-week-old Tac2 knockout (KO) and wild-type (WT) littermates immunostained as in . Scale bar, 50 μm. (B) Number of S100B + glia in each spatial-morphological class of the ileum in Tac2 KO (orange) and WT (gray) mice ( n = 4–5 mice/genotype). (C) Number of ANNA-1 + neurons in each enteric plexus in Tac2 KO and WT mice. (D) Whole-mount preparation from adult Tac2 Cre/+ Rosa26 tdTomato/+ mouse ileum immunolabeled with S100B (green). Scale bar, 100 μm. Graph shows percentage of tdTomato + neurons in the myenteric plexus. (E) Whole-mount preparation of Tac2 Cre/+ Rosa26 tdTomato/+ ileum immunostained for CALB2 (green) and shown at the same scale as (A). (F) Association of TAC3 SNPs with hard stool (top) and human esophagus muscularis eQTLs (bottom) from UK Biobank and GTEx Portal data, respectively. Genomic region analyzed is shown and arrow depicts the direction of TAC3 transcription. For (B) and (C), two-way ANOVA with Šidák’s multiple comparisons test was used (* p < 0.05 and **** p < 0.0001). See also .

Article Snippet: Mouse: Tac2 Cre/+ Strain Background: C57BL/6J , The Jackson Laboratory , RRID: IMSR_JAX:018938.

Techniques: Knock-Out, Immunolabeling

Journal: Neuron

Article Title: Tachykinin signaling defines distinct populations of glia in the enteric nervous system

doi: 10.1016/j.neuron.2025.11.030

Figure Lengend Snippet: (A) Schematic of experimental design. GFP + (glia) and GFP − (non-glia) cells were isolated from the mucosa and muscularis compartments of small intestines from Plp1 eGFP mice. (B) Volcano plots of top 5 significantly up- and downregulated genes in GFP + compared with GFP − cells in total tissue (pooling compartments), muscularis, and mucosal compartments. Dashed line indicates p adj. < 0.05. (C) Volcano plot of macrophage ( Cd300a , Csf1r , Mpeg1 , Cx3cr1 , and Adgre1 ) and glial markers ( S100b , Plp1 , and Gfap ) enriched in mucosal GFP + cells. (D) Csf1r transcripts (yellow) labeled by in situ hybridization in the small intestinal mucosa of a Plp1 CreERT2 Rosa26 tdTomato/+ Cx3cr1 GFP/+ reporter mouse showing glial (tdTomato + ; arrowhead) and macrophage (GFP + ) expression. Scale bar, 10 μm. (E) Proportions of Plp1 tdTomato+ glia (pink) and Cx3cr1 GFP+ macrophages (green) that were labeled by in situ hybridization for putative macrophage transcripts (807 cells, 4 mice). (F) Biological pathways upregulated in mucosal and muscularis glia. (G) Uniform manifold approximation and projection (UMAP) plots of re-analyzed single-cell glial transcriptomes from the CNS and PNS. Heatmap of normalized gene set enrichment scores (NESs) of mucosal and muscularis glia. See also and .

Article Snippet: Mouse: Rosa26 DTA/DTA Strain Background: C57BL/6J , The Jackson Laboratory , RRID: IMSR_JAX:009669.

Techniques: Isolation, Labeling, In Situ Hybridization, Expressing

Journal: Neuron

Article Title: Tachykinin signaling defines distinct populations of glia in the enteric nervous system

doi: 10.1016/j.neuron.2025.11.030

Figure Lengend Snippet: (A) Volcano plots of most significantly upregulated genes in GFP + cells in the muscularis compartment and the subset enriched in muscularis glia. (B) Whole-mount preparations of ileum and distal colon from adult Plp1 eGFP mice with immunohistochemical labeling of TACR3 (magenta) imaged at the myenteric plexus. Insets show magnified view of boxed regions. Arrowheads mark TACR3-immunoreactive GFP + glia surrounding larger GFP − soma (asterisk), presumably neuronal. (C) Schematic illustrating spatial-morphological classification of glia in the small intestine. (D) Schematic and image of the distal colon from a 3-week-old Tacr3 IRES-Cre/+ Rosa26 tdtomato/+ mouse showing tdTomato expression throughout the myenteric plexus (scale bar, 1 mm). (E) Whole-mount preparations of duodenum, ileum, and distal colon from 3-week-old Tacr3 IRES-Cre/+ Rosa26 tdtomato/+ mice imaged at the myenteric plexus. TdTomato expression colocalizes with S100B (cyan) in myenteric glia (arrowheads and arrows mark example intraganglionic and connective glia, respectively) but not extranganglionic glia (asterisks). (F) Tacr3 reporter expression in ANNA-1 + neurons (cyan) in the myenteric (MP) and submucosal (SMP) plexuses. For (E) and (F), n = 5 mice; scale bar, 20 μm; see also . For (E), one-way ANOVA with Tukey’s multiple comparisons test was used (* p < 0.05, ** p < 0.005, **** p < 0.0001, and ns, not significant). For (F), unpaired t test was used.

Article Snippet: Mouse: Rosa26 DTA/DTA Strain Background: C57BL/6J , The Jackson Laboratory , RRID: IMSR_JAX:009669.

Techniques: Immunohistochemical staining, Labeling, Expressing

Journal: Neuron

Article Title: Tachykinin signaling defines distinct populations of glia in the enteric nervous system

doi: 10.1016/j.neuron.2025.11.030

Figure Lengend Snippet: (A) Ileums from 3-week-old Tac2 knockout (KO) and wild-type (WT) littermates immunostained as in . Scale bar, 50 μm. (B) Number of S100B + glia in each spatial-morphological class of the ileum in Tac2 KO (orange) and WT (gray) mice ( n = 4–5 mice/genotype). (C) Number of ANNA-1 + neurons in each enteric plexus in Tac2 KO and WT mice. (D) Whole-mount preparation from adult Tac2 Cre/+ Rosa26 tdTomato/+ mouse ileum immunolabeled with S100B (green). Scale bar, 100 μm. Graph shows percentage of tdTomato + neurons in the myenteric plexus. (E) Whole-mount preparation of Tac2 Cre/+ Rosa26 tdTomato/+ ileum immunostained for CALB2 (green) and shown at the same scale as (A). (F) Association of TAC3 SNPs with hard stool (top) and human esophagus muscularis eQTLs (bottom) from UK Biobank and GTEx Portal data, respectively. Genomic region analyzed is shown and arrow depicts the direction of TAC3 transcription. For (B) and (C), two-way ANOVA with Šidák’s multiple comparisons test was used (* p < 0.05 and **** p < 0.0001). See also .

Article Snippet: Mouse: Rosa26 DTA/DTA Strain Background: C57BL/6J , The Jackson Laboratory , RRID: IMSR_JAX:009669.

Techniques: Knock-Out, Immunolabeling